Derivative Assay:Article Title: Cerulenin partially corrects the disrupted developmental transcriptomic signature in Huntington’s disease striatal medium spiny neurons
Article Snippet: .. This medium contained Neurobasal (Thermo Fisher Scientific, 21103049), 1× B27 supplement (Thermo Fisher Scientific, 17504001), 1× GlutaMAX (Thermo Fisher Scientific, 35050061), leukemia inhibitory factor (10 ng/mL, PeproTech, 300-05), and 100 U/mL P/S, supplemented with 25 ng/mL β-FGF and 25 ng/mL activin A. NSCs derived from this process were passaged upon reaching confluency. .. To produce developing MSNs, culture plates were coated with 1 mL of poly-D-lysine hydrobromide (100 μg/mL, Sigma-Aldrich, P6407) and incubated overnight at 37 °C with 5% CO 2 .
other:Article Title: Reconstructing the emergence of the human chorion via HIPPO-mediated trophoblast induction
Article Snippet: On day 12 of TE induction, cells were transitioned into basal medium : 1:1 mixture of Neurobasal (Gibco):DMEM/F12 without HEPES and L-glutamine (Corning), supplemented with: 1× GlutaMAX (Gibco), 1× MEM non-essential amino acids (Gibco), 1× ITS (Sigma-Aldrich), 1× B27 without vitamin A (Gibco), 1× β-mercaptoethanol (Sigma-Aldrich), 1× Antibiotic-Antimycotic (Gibco), 50 μg/mL L-ascorbic acid (Sigma-Aldrich), and 0.2% (v/v) Cultrex (R&D Systems), 1 μM Progesterone (Sigma-Aldrich), and 10 nM Estrogen (Sigma-Aldrich) ± 50 ng/ml EGF (Sino Biological) and 2 μM CHIR99021(ApexBio).
Article Title: Impaired BDNF-TrkB trafficking and signalling in Down syndrome basal forebrain neurons
Article Snippet: Two hours after plating, medium was changed to maintenance medium (Neurobasal; Gibco) supplemented with 1% penicillin/streptomycin (ThermoFisher), 38 mM NaCl, 0.6% glucose, 1× GlutaMAX, 1× B27 (Gibco), 10 ng/mL NGF and 10 ng/mL BDNF).
Article Title: Reconstructing the emergence of the human chorion via HIPPO-mediated trophoblast induction
Article Snippet: Composition, basal medium : 1:1 mixture of Neurobasal (Gibco):DMEM/F12 without HEPES and L-glutamine (Corning), supplemented with: 1× GlutaMAX (Gibco), 1× MEM non-essential amino acids (Gibco), 1× ITS (Sigma-Aldrich), 1× B27 without vitamin A (Gibco), 1× β-mercaptoethanol (Sigma-Aldrich), 1× Antibiotic-Antimycotic (Gibco), 50 μg/mL L-ascorbic acid (Sigma-Aldrich), and 0.2% (v/v) Cultrex (R&D Systems); + HENSM active ingredients (slightly optimized from published composition) : 20 ng/mL rhLIF (Sino Biological), 2 μM XAV939 (Cayman Chemical Company), 1 μM PD0325901 (Cayman Chemical Company), 2 μM Gö6983 (Cayman Chemical Company), 1.2 μM CGP77675 (Cayman Chemical Company), 10 ng/mL Activin A (Sino Biological), and 1.2 μM Y-27632); + 2i : 0.5 mM GSK-3484862 (DNMT1 inhibitor, ChemieTek) and 50nM DZNep (EZH2 inhibitor, Selleck Chemicals).
Article Title: Reconstructing the emergence of the human chorion via HIPPO-mediated trophoblast induction
Article Snippet: TA medium composition, basal medium : 1:1 mixture of Neurobasal (Gibco):DMEM/F12 without HEPES and L-glutamine (Corning), supplemented with: 1× GlutaMAX (Gibco), 1× MEM non-essential amino acids (Gibco), 1× ITS (Sigma-Aldrich), 1× B27 without vitamin A (Gibco), 1× β-mercaptoethanol (Sigma-Aldrich), 1× Antibiotic-Antimycotic (Gibco), 50 μg/mL L-ascorbic acid (Sigma-Aldrich), and 0.2% (v/v) Cultrex (R&D Systems) + active ingredients : 3 μM TDI-011536 (MedChem Express) ± 1 μM A83-01 (Cayman Chemical Company) for the first 6 days (see main text).
Generated:Article Title: Structural basis for the synergistic assembly of the snRNA export complex.
Article Snippet: The data were analyzed and mass estimated automatically using the DiscoverMP software (v2024 R1, Refeyn). .. All mES cell lines used or generated in this study were descendants of wildtype ES-E14TG2a cells (male genotype, XY). mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF (leukemia inhibitory factor) containing media (1:1 mix of DMEM/F12 (Thermo Fisher) and Neurobasal (Thermo Fisher) supplemented with 1% (v/v) penicillin– streptomycin (Sigma), 2 μM of Glutamax (Thermo Fisher), 0.1 mM of nonessential amino acids (Thermo Fisher), 1 mM of sodium pyruvate (Thermo Fisher), 0.5x N-2 Supplement (Thermo Fisher), 0.5x B-27 Supplement (Thermo Fisher), 50 μM of 2-mercaptoethanol (Thermo Fisher), 3 μM of GSK3-inhibitor (CHIR99021, Sigma), 1 μM of MEK-inhibitor (PD0325901, Sigma) and LIF (produced in house)) at 37 °C and 5% CO2. .. Cells were passaged every 48–72 h by dissociating cells with 0.05% trypsin (Sigma) before neutralizing with an equal volume of 1x trypsin inhibitor (Thermo Fisher).
Cell Culture:Article Title: Structural basis for the synergistic assembly of the snRNA export complex.
Article Snippet: The data were analyzed and mass estimated automatically using the DiscoverMP software (v2024 R1, Refeyn). .. All mES cell lines used or generated in this study were descendants of wildtype ES-E14TG2a cells (male genotype, XY). mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF (leukemia inhibitory factor) containing media (1:1 mix of DMEM/F12 (Thermo Fisher) and Neurobasal (Thermo Fisher) supplemented with 1% (v/v) penicillin– streptomycin (Sigma), 2 μM of Glutamax (Thermo Fisher), 0.1 mM of nonessential amino acids (Thermo Fisher), 1 mM of sodium pyruvate (Thermo Fisher), 0.5x N-2 Supplement (Thermo Fisher), 0.5x B-27 Supplement (Thermo Fisher), 50 μM of 2-mercaptoethanol (Thermo Fisher), 3 μM of GSK3-inhibitor (CHIR99021, Sigma), 1 μM of MEK-inhibitor (PD0325901, Sigma) and LIF (produced in house)) at 37 °C and 5% CO2. .. Cells were passaged every 48–72 h by dissociating cells with 0.05% trypsin (Sigma) before neutralizing with an equal volume of 1x trypsin inhibitor (Thermo Fisher).
Produced:Article Title: Structural basis for the synergistic assembly of the snRNA export complex.
Article Snippet: The data were analyzed and mass estimated automatically using the DiscoverMP software (v2024 R1, Refeyn). .. All mES cell lines used or generated in this study were descendants of wildtype ES-E14TG2a cells (male genotype, XY). mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF (leukemia inhibitory factor) containing media (1:1 mix of DMEM/F12 (Thermo Fisher) and Neurobasal (Thermo Fisher) supplemented with 1% (v/v) penicillin– streptomycin (Sigma), 2 μM of Glutamax (Thermo Fisher), 0.1 mM of nonessential amino acids (Thermo Fisher), 1 mM of sodium pyruvate (Thermo Fisher), 0.5x N-2 Supplement (Thermo Fisher), 0.5x B-27 Supplement (Thermo Fisher), 50 μM of 2-mercaptoethanol (Thermo Fisher), 3 μM of GSK3-inhibitor (CHIR99021, Sigma), 1 μM of MEK-inhibitor (PD0325901, Sigma) and LIF (produced in house)) at 37 °C and 5% CO2. .. Cells were passaged every 48–72 h by dissociating cells with 0.05% trypsin (Sigma) before neutralizing with an equal volume of 1x trypsin inhibitor (Thermo Fisher).
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